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Table 2 Repression of LacZ activity in T. reesei d-glucose and glycerol cultures

From: l-Methionine repressible promoters for tuneable gene expression in Trichoderma reesei

 

Basal

l-Methionine

l-Cysteine

1 mM

5 mM

1 mM

5 mM

Glucose

 P 123979 -lacZ 3

1.27 ± 0.15

0.04 ± 0.01

0.00

1.02 ± 0.07

0.00

 P 123979 -lacZ 7

1.46 ± 0.12

0.05 ± 0.00

0.00

0.81 ± 0.31

0.00

 QM9414

0.00

    

Glycerol

 P 123979 -lacZ 3

1.09 ± 0.08

0.09 ± 0.02

0.03 ± 0.00

n.d

n.d

 P 123979 -lacZ 7

1.02 ± 0.59

0.10 ± 0.00

0.00

n.d

n.d

 QM9414

0.05 ± 0.00

    
  1. Intracellular LacZ activity relative to an equally treated constitutively expressing lacZ strain (P tef1 -lacZ).
  2. n.d. activity not determined.