Fig. 3From: Functional analysis of filipin tailoring genes from Streptomyces filipinensis reveals alternative routes in filipin III biosynthesis and yields bioactive derivativesConstruction of cytochrome P450 mutants. a Predicted PCR fragment amplification of the parental strain and the different mutants. The primers used in the assay are indicated with arrowheads. The acc(3)IV-oriT cassette is indicated in black. b–d PCR analysis of the wild type and the mutants.Back to article page