Mapping and analysis of Mth1 mutations. Magenta text indicates positions for non-synonymous mutations identified in this study. a Result of a multiple alignment using homologous sequences (n = 22). The Mth1 sequence of S. cerevisiae is shown with colored conservation levels. Yellow indicates low conservation, white intermediate, and blue high. Gray and italic text indicates the phosphorylation region of casein kinase Yck1. b Analysis of the 22 amino acids from the conserved region at position 70–91 and secondary structure predictions using six different prediction programs [25, 26]. c A helix wheel representation of amino acids predicted to form an alpha-helix (codon 75–89). The amino acid types are colored as in b.