An artificial neural network for membrane-bound catechol-O-methyltransferase biosynthesis with Pichia pastoris methanol-induced cultures

Table 1 Time course profile of the biomass levels (measured as OD_{600 nm}) obtained in the trials where the methanol constant feed flow-rate (2, 3.6 and 5.2 mL/L/H) and the DMSO levels added to the culture were changed, in accordance with the results shown in Fig. 2b

Time after induction phase (h)	Optical density measurements at 600 nm
	Methanol constant feed flow-rate			DMSO concentration
	2 mL/L/H	3.6 mL/L/H	5.2 mL/L/H	2.5% (v/v)	5% (v/v)	7.5% (v/v)
3	111.75 ± 1.23	105.19 ± 5.57	116.75 ± 4.42	112.75 ± 4.95	113.44 ± 4.33	104.88 ± 2.47
9	110.32 ± 2.38	106.88 ± 7.95	110.5 ± 3.36	110.19 ± 2.21	114.43 ± 1.17	113.44 ± 0.27
15	111.31 ± 4.68	111.5 ± 9.02	117.38 ± 2.47	116.31 ± 2.21	132.00 ± 7.07	115.06 ± 3.62

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