Skip to main content

Table 3 Strains and plasmids used in this study

From: Modification of targets related to the Entner–Doudoroff/pentose phosphate pathway route for methyl-d-erythritol 4-phosphate-dependent carotenoid biosynthesis in Escherichia coli

Strain/plasmid

Description

References

Escherichia coli strains

 BW25113

Wild type K-12 strain

Our lab

 DH5αa

For genetic manipulation

Invitrogen

 TX

PT5-dxs K-12 strain, for construction of strains that harboring PT5-dxs phenotype

This study

 TI

PT5-idi K-12 strain, for construction of strains that harboring PT5-idi phenotype

This study

 GRK

galR::P119-glk K-12 strain, for construction of strains that harboring galR::P119-glk phenotype

This study

 W036

BW25113, pLY036 plasmid

This study

 P036

Δpgi, pLY036 plasmid

This study, KEIO

 KA036

ΔpfkA, pLY036 plasmid

This study, KEIO

 KB036

ΔpfkB, pLY036 plasmid

This study, KEIO

 KAB036

ΔpfkAΔpfkB, pLY036 plasmid

This study

 F036

ΔfbaB, pLY036 plasmid

This study, KEIO

 W-AZ036

BW25113, pBAD-zwf and pLY036 plasmids

This study

 W-AE036

BW25113, pBAD-eda and pLY036 plasmids

This study

 W-AD036

BW25113, pBAD-edd and pLY036 plasmids

This study

 TX036

PT5-dxs, pLY036 plasmid

This study

 PTX036

ΔpgiPT5-dxs, pLY036 plasmid

This study

 W-SX036

BW25113, pSB1s-dxs and pLY036 plasmids

This study

 P-SX036

Δpgi, pSB1s-dxs and pLY036 plasmids

This study, KEIO

 TX-SX036

PT5-dxs, pSB1s-dxs and pLY036 plasmids

This study

 PTX-SX036

Δpgi PT5-dxs, pSB1s-dxs and pLY036 plasmids

This study

 P-AE036

Δpgi, pBAD-eda and pLY036 plasmids

This study, KEIO

 P-A-SEX036

Δpgi, pBAD, pSB1s-eda-dxs and pLY036 plasmids

This study, KEIO

 P-AE-SX036

Δpgi, pBAD-eda, pSB1s- dxs and pLY036 plasmids

This study, KEIO

 P-AS-SEX036

Δpgi, pBAD-SS, pSB1s-eda-dxs and pLY036 plasmids

This study, KEIO

 PD-SX036

ΔpgiΔedd, pSB1s-dxs and pLY036 plasmids

This study

 PG-SX036

ΔpgiΔgnd, pSB1s-dxs and pLY036 plasmids

This study

 P-AEG036

Δpgi, pBAD-eda-gnd and pLY036 plasmids

This study, KEIO

 P-AEGG036

Δpgi, pBAD-eda-GCgnd and pLY036 plasmids

This study, KEIO

 PG036

ΔpgiΔgnd, pLY036 plasmid

This study

 PG-AE036

ΔpgiΔgnd, pBAD-eda and pLY036 plasmids

This study

 PG-AEG036

ΔpgiΔgnd, pBAD-eda-gnd and pLY036 plasmids

This study

 PG-AEGG036

ΔpgiΔgnd, pBAD-eda-GCgnd and pLY036 plasmids

This study

 W-SXID036

BW25113, pSB1s-dxs-idi-ispDF and pLY036 plasmids

This study

 P-SXID036

Δpgi, pSB1s-dxs-idi-ispDF and pLY036 plasmids

This study, KEIO

 KA-SXID036

ΔpfkA, pSB1s-dxs-idi-ispDF and pLY036 plasmids

This study

 KAB-SXID036

ΔpfkAΔpfkB, pSB1s-dxs-idi-ispDF and pLY036 plasmids

This study

 PKAB-SXID036

ΔpgiΔpfkAΔpfkB, pSB1s-dxs-idi-ispDF and pLY036 plasmids

This study

 PF-SXID036

ΔpgiΔfbaB, pSB1s-dxs-idi-ispDF and pLY036 plasmids

This study

 PY-SXID036

ΔpgiΔytjC, pSB1s-dxs-idi-ispDF and pLY036 plasmids

This study

 PTXI036

Δpgi PT5-dxs PT5-idi, pLY036 plasmids

This study

 PTXI-SXID036

Δpgi PT5-dxs PT5-idi, pSB1s-dx -idi-ispDF and pLY036 plasmids

This study

 W10RK

BW25113, pLY10RK plasmid

This study

 TXI-SXID10RK

PT5-dxs PT5-idi, pSB1s-dx -idi-ispDF and pLY10RK plasmids

This study

 PTXI-SXID10RK

Δpgi PT5-dxs PT5-idi, pSB1s-dx -idi-ispDF and pLY10RK plasmids

This study, KEIO

 PTXIGK-AEGG-SXID10RK

Δpgi, PT5-dxsPT5-idi, ΔptsGgalR::P119-glk, pBAD-eda-GCgnd, pSB1s-dxs-idi-ispDF and pLY10RK plasmids

This study, KEIO

Other bacterial strains

 Anabaena sp. PCC7120

crtE, crtB for pLY036

Our lab

 Rhodobacter sphaeroides 2.4.1

crtI for pLY036

Our lab

 Pantoea ananatis

crtB. crtI for pLY10RK

Our lab

 Pantoea agglomerans

crtE for pLY10RK

Our lab

Plasmids

 pKD46

For lambda-Red mediated recombination

[25]

 pUKM

For lambda-Red mediated recombination, derived from pKD4 by inserting multiple cloning site closed to FRT

Our lab

 pCP20

For lambda-Red mediated recombination

[25]

 pUKM-T5

For construction of TX and TI, derived from pUKM by inserting T5 promoter at multiple cloning site

This study

 pBAD-HisA

ColE1 origin, araBAD promoter, AmpR

Invitrogen

 pSB1s

pSC101 origin, araBAD promoter, StrR

Our lab

 pS95s

pSC101 origin, constitutive P119 promoter (derived from iGEM part BBa_J23119), StrR

Our lab

 pS95s-glk

pSC101 origin, constitutive P119 promoter, StrR,E. coli glk, for construction of pUKM-glk

This study

 pUKM-glk

For construction of GRK, derived from pUKM by inserting P119-glk fragment at multiple cloning site

This study

 pLY036

p15A origin, araBAD promoter, CmR, Anabaena sp crtEB, R. sphaeroides crtI, E. coli idi

This study

 pLY10RK

RSF1030 origin, araBAD promoter, KanR, P. agglomerans crtE, P. ananatis crtBI, E. coli idi

This study

 pSB1s-dxs

pSC101 origin, araBAD promoter, StrR,E. coli dxs

This study

 pSB1s-eda-dxs

pSC101 origin, araBAD promoter, StrR, dockerin-fused E. coli eda, dockerin-fused E. coli dxs

This study

 pSB1s-dxs-idi-ispDF

pSC101 origin, araBAD promoter, StrR, E. coli dxs,idi,ispDF

This study

 pBAD-zwf

ColE1 origin, araBAD promoter, AmpR, E. coli zwf

This study

 pBAD-edd

ColE1 origin, araBAD promoter, AmpR, E. coli edd

This study

 pBAD-eda

ColE1 origin, araBAD promoter, AmpR, E. coli eda

This study

 pBAD-eda-gnd

ColE1 origin, araBAD promoter, AmpR, E. coli eda,gnd

This study

 pBAD-eda-GCgnd

ColE1 origin, araBAD promoter, AmpR, E. coli eda,GCtag-gnd

This study

 pBAD-SS

ColE1 origin, araBAD promoter, AmpR, clostridium sp cellulose scaffold protein

This study