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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Functionalized PHB granules provide the basis for the efficient side-chain cleavage of cholesterol and analogs in recombinant Bacillus megaterium

Fig. 5

Effect of PHA polymerase subunit phaC knockout on morphology of strain GHH3. a Nile red stained ΔphaC strain GHH3 exhibits no accumulation of PHB. b PHB production is restored in cells after transformation with phaC containing plasmid pSMF3_RBCP. c Strain GHH3 transformed with pSMF2.1_CYP11A1eGFP. Expressed CYP11A1eGFP forms aggregates in the cell. d, e Expression of phaPeGFP in GHH1 and GHH3, respectively, both stained with Nile red (overlay). f Fluorescent probe 25-NBD cholesterol is not stored in cells of strain GHH3 lacking PHB.

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