Fig. 2

Localization of eGFP fused CYP11A1, AdR and Adx as well as 25-NBD cholesterol in cells of B. megaterium strain MS941 analyzed by confocal fluorescence microscopy. a–c CYP11A1eGFP, AdReGFP and AdxeGFP expressing cells, respectively, counterstained with FM 4-64. CYP11A1eGFP and AdReGFP form aggregates, while AdxeGFP is expressed in a soluble form. d Staining of untransformed cells with PHB specific dye Nile red. e–g Nile red staining of cells expressing CYP11A1eGFP, AdReGFP and AdxeGFP, respectively (merged images). From left to right: eGFP emission signal, Nile red emission signal, and overlay, showing overlapping signals in yellow. CYP11A1eGFP and AdReGFP are localized at the PHB granules, while AdxeGFP is present solely in the cytosol. h 25-NBD cholesterol accumulated in PHB bodies of cells. The number of single cells attached to one another via their peptidoglycane layer differs, leading to differently sized cell aggregates.