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Table 1 Summary of the conditions used in fermenter runs (FR)

From: Acetate: friend or foe? Efficient production of a sweet protein in Escherichia coli BL21 using acetate as a carbon source

 

FR1

FR2

FR3

FR4

Sodium acetate

0.4%

0.4%

0.4%

1%

Starting pH

7.0

7.0

8.0

8.0

pO2

20%

10%

10%

10%

pH control

No

No

Yes

Yes

Maximum OD600/mL

1.3 ± 0.1

1.9 ± 0.1

3.2 ± 0.1

5.0 ± 0.3

μmax (h−1)

0.7

0.9

1.0

1.0

DCW (g/L)

0.7 ± 0.1

1.0 ± 0.1

1.7 ± 0.1

2.7 ± 0.2