Figure 6From: Acetate: friend or foe? Efficient production of a sweet protein in Escherichia coli BL21 using acetate as a carbon sourceComparison of the CD spectra of the proteins from fermentation in PYA8 and in LB. Mean residue ellipticity of MNEI obtained from representative fermenter runs (FR2, blue curve; FR3, red curve; FR4, yellow curve; LB control expression, green curve). CD spectra are indicative of the typical fold of MNEI, characterized by abundance of β-sheet secondary structures. The spectra do not show any significant difference when the protein is purified from cultures in acetate containing medium or LB.Back to article page