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Table 3 Effect of putA deletion, p4h1of expression, proline addition, and proline hydroxylation on NTP and cofactor formation and consumption rates

From: Metabolic network capacity of Escherichia coli for Krebs cycle-dependent proline hydroxylation

 

wt_pET

ΔputA_pET

wt_p4h1of

ΔputA_p4h1of

−Pro

+Pro

−Pro

+Pro

−Pro

+Pro

−Pro

+Pro

Catabolisma

 NADPH

6.4

8.0

6.8

6.8

6.6

6.2

6.4

4.7

 NADH

17.8

19.1

18.5

17.7

19.2

17.6

19.3

12.9

 FADH2

2.1

3.7

2.5

2.5

3.0

2.9

3.1

1.7

 ATP/GTP

14.1

14.5

14.3

13.4

14.1

13.3

14.3

10.1

Biomass formation

 NADPH

−7.3

−7.9

−7.0

−7.3

−5.7

−5.7

−5.2

−5.4

 NADH

1.2

1.3

1.1

1.2

0.9

0.9

0.8

0.8

 FADH2

 ATP

−7.3

−8.1

−7

−7.3

−5.4

−5.4

−4.9

−5.1

Transhydrogenation

 NADPH

0.9

−0.1

0.2

0.5

−0.9

−0.5

−1.2

0.7

 NADH

−0.9

0.1

−0.2

−0.5

0.9

0.5

1.2

−0.7

Oxidative phosphorylation

 NADPH

 NADH

−18.1

−20.5

−19.4

−18.4

−21.0

−19.0

−21.3

−13.0

 FADH2

−2.1

−3.7

−2.5

−2.5

−3.0

−2.9

−3.1

−1.7

 ATP

58.4

67.3

63.3

60.1

68.9

62.3

70.0

42.5

Maintenanceb

 ATP

−65.2

−73.7

−70.6

−66.2

−77.6

−70.2

−79.4

−47.5

  1. E. coli BL21(DE3)(pLysS) strains were grown in M9 medium containing 5 g L−1 glucose and optionally 5 mM proline (Pro) at 30°C and 250 rpm. Rates are given in mmol g −1CDW h−1. wt_pET,  E. coli BL21(DE3)(pLysS)(pET-24a); ΔputA_pET, E. coli BL21ΔputA(DE3)(pLysS)(pET-24a); wt_p4h1of, E. coli BL21(DE3)(pLysS)(pET_p4h1of); ΔputA_p4h1of, E. coli BL21ΔputA(DE3)(pLysS)(pET_p4h1of)
  2. aThe calculations were based on the reactions described in Additional file 1: Table S5 and included in the metabolic map presented in Fig. 2.
  3. bThe ATP costs for maintenance were not divided into growth and non-growth associated maintenance.