Metabolic network capacity of Escherichia coli for Krebs cycle-dependent proline hydroxylation

Table 2 Fractional abundance of unlabeled amino acid fragments (m0) during cultivation on labeled glucose with and without unlabeled proline

	wt_pET		ΔputA_pET		wt_p4h1of		ΔputA_p4h1of
	−Pro	+Pro	−Pro	+Pro	−Pro	+Pro	−Pro	+Pro
Glycolysis
Ala [M-57]⁺	0.366	0.377	0.366	0.368	0.365	0.360	0.361	0.359
Gly [M-57]⁺	0.622	0.624	0.621	0.622	0.621	0.621	0.619	0.620
Val [M-57]⁺	0.175	0.183	0.176	0.175	0.174	0.169	0.169	0.165
Leu [M-159]⁺	0.113	0.12	0.114	0.114	0.113	0.105	0.109	0.104
Ser [M-57]⁺	0.319	0.322	0.321	0.321	0.313	0.311	0.313	0.310
Phe [M-57]⁺	0.111	0.114	0.111	0.110	0.113	0.109	0.109	0.108
TCA
Asp [M-57]⁺	0.195	0.336	0.194	0.194	0.191	0.233	0.187	0.179
Thr [M-57]⁺	0.193	0.331	0.190	0.190	0.187	0.232	0.184	0.176
Ile [M-159]⁺	0.140	0.225	0.140	0.140	0.137	0.157	0.135	0.128
Glu [M-57]⁺	0.115	0.333	0.115	0.115	0.113	0.195	0.112	0.105
Pro [M-159]⁺	0.184	0.818	0.181	0.819	0.176	0.819	0.175	0.820

Cells were grown aerobically at 30°C in M9 medium with 5 g L⁻¹ labeled glucose (80% 1-¹³C and 20% U-¹³C) in the presence (+Pro) and absence (−Pro) of 5 mM unlabeled proline. wt_pET, E. coli BL21(DE3)(pLysS)(pET-24a); ΔputA_pET, E. coli BL21ΔputA(DE3)(pLysS)(pET-24a); wt_p4h1of, E. coli BL21(DE3)(pLysS)(pET_p4h1of); ΔputA_p4h1of, E. coli BL21ΔputA(DE3)(pLysS)(pET_p4h1of).

ISSN: 1475-2859