Fig. 3From: Metabolic network capacity of Escherichia coli for Krebs cycle-dependent proline hydroxylationIntracellular a-ketoglutarate (a-KG) and succinate (Succ) concentrations in the mid-exponential growth phase for recombinant E. coli BL21(DE3)(pLysS) (wt) and E. coli BL21ΔputA(DE3)(pLysS) (ΔputA) containing pET-24a (pET) or pET_p4h1of (p4h1of) in M9 medium with 5 g L−1 glucose (Glc) in the absence or presence of 5 mM proline (Pro) at 30°C. Concentrations are given as average values in µmoles per gram cell dry weight from three different samples with standard deviations displayed as error bars.Back to article page