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Table 4 Model calibrated parameters with description and simulation values

From: A novel process-based model of microbial growth: self-inhibition in Saccharomyces cerevisiae aerobic fed-batch cultures

Symbol

Description

Unit

Calibration starting value

Calibrated values

LBG H 1022a

CBS 8066b

CEN.PK113-7Dc

CEN.PK2-1Cd

v G

Maximum uptake rate

h−1

3.64 [54]

3.3

5.1

5.8

5.8

k G

Uptake saturation constant

g l−1

0.18 [55]

0.2

0.2

0.27

0.27

η G

Uptake efficiency P/G

–

0.92 [56]

0.76

0.76

0.86

0.64

v RP

Maximum glycolysis products respiration rate

h−1

0.475 [57]

1.5

0.67

1.0

0.83

k RP

Glycolysis products respiration saturation constant

g l−1

0.033–0.035 [58]

0.24

0.21

0.24

0.18

η RP

Respiration efficiency C M /P

–

0.6 [56]

0.80

0.80

0.80

0.73

v F

Maximum fermentation rate

h−1

11.8 [57]

3.3

4.17

2.6

6.57

k F

Fermentation saturation constant

g l−1

0.5 [59]

0.13

0.18

0.14

0.16

η FE

Fermentation efficiency E/P

–

0.47 [60]

0.595

0.80

0.60

0.61

η FP

Fermentation efficiency C M /P

–

0.09 [61]

0.13

0.16

0.20

0.10

v RE

Maximum ethanol respiration rate

h−1

0.19 [11]

0.20

0.14

0.20

0.11

k RE

Ethanol respiration saturation constant

g l−1

0.1 [13]

0.12

0.12

0.15

0.15

η RE

Respiration efficiency C M /E

–

0.68 [62]

0.65

0.55

0.80

0.80

v A

Maximum accumulation rate

h−1

0.2 (Arbitrary)

0.2

0.2

0.2

0.3

k A

Accumulation saturation constant

g l−1

0.05 (Arbitrary)

0.05

0.05

0.05

0.03

η A

Accumulation efficiency R/P

–

0.2 (Arbitrary)

0.2

0.2

0.2

0.2

r MAX

Maximum reserves/cell mass ratio

–

0.25 [63]

0.3

0.3

0.3

0.3

δ

Death rate

h−1

0.017–0.032 [27]

0.05

0.05

0.05

0.1

τ

Death threshold

g l−1

0.6 (Arbitrary)

0.6

0.6

0.6

0.6

ρ

Secretion rate

h−1

0.01 (Arbitrary)

0.01

0.01

0.01

0.02

σ I

Sensitivity to inhibitor NF

–

1.0 (Arbitrary)

1.0

1.0

1.0

1.68

σ E

Sensitivity to ethanol NF

–

1.4 [49, 64, 65]

1.4

1.4

1.4

1.4

c

Cell volume/dry weight ratio

l g−1

0.01 [66]

0.01

0.01

0.01

0.01

a

Metabolic switches calibration parameter

–

2.0 × 10−4 (Arbitrary)

2.0 × 10−4

2.0 × 10−4

2.0 × 10−4

2.0 × 10−4

b

Metabolic switches calibration parameter

l g−1

30 (Arbitrary)

30

30

30

30

l 1

Lag phase calibration parameter

–

0.58 (Arbitrary)

–

0.58

–

–

l 2

Lag phase calibration parameter

–

2.0 × 10−5 (Arbitrary)

–

2.0 × 10−5

–

–

l 3

Lag phase calibration parameter

h−1

5.8 (Arbitrary)

–

5.8

–

–

[P] MAX

Glycolysis products maximum concentration

g l−1

1 [67, 68]

1

1

1

1

[E] MAX

Ethanol maximum concentration

g l−1

100 [65]

100

100

100

100

[I] MAX

Inhibitor maximum concentration

g l−1

1 (Arbitrary)

1

1

1

1

  1. avon Meyenburg [53]; Fig. 2.
  2. bPham et al. [12]; Fig. 3.
  3. cFigure 4.
  4. dFigures 5, 6 and 7.
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Microbial Cell Factories

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