Figure 3

Type of tag and position (N/C-terminal) strongly affect volumetric HRP activities. Volumetric HRP activities in the supernatant with ABTS as substrate after cultivation on methanol for 72 h are shown. The pPpRSFC plasmids used are indicated via the numbers given in Table 1, the use of a signal sequence (MFalpha), different promoter than pAOX1 and if applicable tag and position (N/C) are given. Mean values and standard deviations of biological triplicates are shown.