Figure 2

Representative map of P. pastoris RSFC plasmids. The features of all RSFC plasmids designed are summarized in this schematic map. Different promoters, N/C-terminal tags, resistance markers are shown. pGAP plasmids do not contain the 3′ AOX1 homologous sequence for recombination (3′ AOX1 HS). HIS4 vectors contain in addition an ampicillin resistance cassette. The mating factor alpha signal sequence (MF alpha) is optional and only present in secretory plasmids. See Table 1 for the part combinations created in this study. Features are not drawn to scale. Exact plasmid maps are provided in the Additional file 3 in GenBank format.