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Table 6 Strain characteristic parameters during a glycerol pulse in sorbitol fed-batch FB3 (glycerol was pulsed at a final concentration of 10 g L−1 to the culture while sorbitol was fed at a constant qs sorbitol = 0.197 g g−1 h−1)

From: Development of a mixed feed strategy for a recombinant Pichia pastoris strain producing with a de-repression promoter

qs glycerol (g g−1 h−1)

qs sorbitol (g g−1 h−1)

µ (h−1)

\({\text{Y}}_{{{\text{CO}}_{2} /{\text{S}}}}\) (mol Cmol−1)

YX/S (Cmol Cmol−1)

C-balance

rp (U L−1 h−1)

qp (U g−1 h−1)

0.193

0.071

0.186

0.26

0.71

0.97

0*

0*

  1. * We even measured less active PLC after the glycerol pulse than before, indicating product instability.