Development of a mixed feed strategy for a recombinant Pichia pastoris strain producing with a de-repression promoter

Table 2 Summary of strain specific parameters, metabolites and STY during dynamic batch experiment with substrate pulses as well as economic aspects

C-source	q_s (g g⁻¹ h⁻¹)	\({\text{Y}}_{{{\text{CO}}_{2} /{\text{S}}}}\) (mol Cmol⁻¹)	Y_X/S (Cmol Cmol⁻¹)	C-balance	Metabolites	r_p (U L⁻¹ h⁻¹) per pulse	Price (€ kg⁻¹)
Glucose (batch)	0.059	0.53	0.498	1.03	Acetate (3.0 g L⁻¹) Ethanol (0.6 g L⁻¹)	6.2
Glucose	0.048	0.93	0.004	0.94	Ethanol (0.3 g L⁻¹)	4.2	40
Sorbitol	0.037	0.94	0.002	0.94	nd	4.8	20
Mannose	0.051	0.93	0.008	0.94	ethanol (1.0 g L⁻¹)	4.7	2,000
Fructose	0.048	0.90	0.036	0.94	nd	2.2	62
Maltose	0.006	0.93	0.014	0.94	nd	5.4	252
Glycerol	0.023	1.04	0.011	1.05	nd	4.3	26
Lactic acid	0.005	1.04	0.000	1.04	nd	0.5	24

q _s specific substrate uptake rate, \({\text{Y}}_{{{\text{CO}}_{2} /{\text{S}}}}\) and Y _X/S yields of CO₂ and biomass, C-balance sum of \({\text{Y}}_{{{\text{CO}}_{2} /{\text{S}}}}\)and Y_X/S which should ideally give 1.0, r _p volumetric productivity per pulse and nd none determined.

ISSN: 1475-2859