Table 1 Strains and plasmids
From: Deoxycytidine production by a metabolically engineered Escherichia coli strain
Strain or plasmid | Descriptiona | Source or reference |
|---|---|---|
Strains | ||
 BL21(DE3) | F− ompT hsdS B (r − B m − B ) gal dcm me131 (DE3) | Invitrogen |
 XL1-Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacI q ZΔM15 Tn10 (Tetr)] | Stratagene |
 HLC001 | BL21 ΔdeoA | This study |
 HLC003 | BL21 ΔdeoA Δudp ΔdeoD | This study |
 HLC004 | BL21 ΔdeoA Δudp ΔdeoD Δdcd | This study |
 HLC005 | BL21 ΔdeoA Δudp ΔdeoD Δdcd Δcdd | This study |
 HLC006 | BL21 ΔdeoA Δudp ΔdeoD Δdcd Δcdd ΔcodA | This study |
 HLC007 | BL21 ΔdeoA Δudp ΔdeoD Δdcd Δcdd ΔcodA ΔthyA | This study |
 HLC010 | BL21 ΔdeoA Δudp ΔdeoD Δdcd Δcdd ΔcodA ΔthyA ΔpurR ΔpepA ΔargR | This study |
 HLC015 | BL21 ΔdeoA Δudp ΔdeoD Δdcd Δcdd ΔcodA ΔthyA ΔpurR ΔpepA ΔargR Δpgi | This study |
Plasmids | ||
 pETDuet | ColE1 replicon, bla | Novagen |
 pKD3 | Template plasmid, derivative of pANTSγ, FRT-flanked cat | [43] |
 pKD20 | λ Red helper plasmid, derivative of pINT-ts, araC-P araB and γ β exo DNA fragments | [43] |
 pCP20 | bla and cat, ori TS, thermal inducible FRT recombinase | [43] |
 pETNG | pETDuet T4 nrdCAB operon and pyrG expression under tac promoter, bla | This study |
 pETNY | pETDuet T4 nrdCAB operon and yfbR expression under tac promoter, bla | This study |
 pETGY | pETDuet pyrG operon and yfbR expression under tac promoter, bla | This study |
 pETNGY | pETDuet T4 nrdCAB operon, pyrG and yfbR expression under tac promoter, bla | This study |