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Figure 3 | Microbial Cell Factories

Figure 3

From: Deoxycytidine production by a metabolically engineered Escherichia coli strain

Figure 3

Transcriptional change of pyrimidine and arginine biosynthetic genes by disruption of CarAB repressors (PurR, PepA and ArgR). The numbers are the ratios of the expression levels in HLC010 vs. HLC007. The shaded numbers indicate significantly up-regulated genes in HLC010 (P < 0.05). a The transcriptional change of genes for precursor supply and pyr operon. α-ketoglutarate and oxaloacetate introduced from the TCA cycle might be converted quickly into carbamoyl phosphate and aspartate. The resulting increased precursors can enhance the production of dCyd. b The transcriptional change of the arg operon. Most of the Arg biosynthetic genes were up-regulated except for argG. The decrease of argG transcription may be the rate-limiting step for synthesizing arginine. AKG α-ketoglutarate, OAA oxaloacetate, GLU l-glutamate, GLN l-glutamine, CarP carbamoyl phosphate, ASP l-aspartate, C-ASP carbamoyl aspartate, ORO orotate, OMP orotate mono phosphate, NAG N-acetyl glutamate, NAGP N-acetyl glutamate phosphate, NAGsA N-acetyl glutamate semi aldehyde, NAO N-acetyl ornithine, ORN ornithine, CITR citrulline, Arg-Suc argino succinate, ARG l-arginine.

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