Figure 2

Deoxycytidine degradation assay. To eliminate possible degradation of dCyd which was produced during fermentation, known pyrimidine nucleoside degradation enzymes were disrupted. The disruption of deoA, udp and deoD, which are active on deoxyuridine and thymidine, was not sufficient to block dCyd degradation completely. However, after the disruption of both dcd and cdd, dCyd degradation was not observed in the in vitro assay. Negative control with dCyd (open circle), BL21(DE3) (filled circle), HLC001 (ΔdeoA) (open square), HLC003 (ΔdeoA ΔdeoD Δudp) (filled square), HLC004 (ΔdeoA ΔdeoD Δudp Δdcd) (open triangle) and HLC005 (ΔdeoA ΔdeoD Δudp Δdcd Δcdd) (filled triangle).