Skip to main content

Table 3 Method for plasmid construction

From: Surface display of an anti-DEC-205 single chain Fv fragment in Lactobacillus plantarum increases internalization and plasmid transfer to dendritic cells in vitro and in vivo

Plasmid name

Original plasmid

References

Restriction sites

Primer used for aDEC amplification

pLip-aDec

pLp_1452Inv

[31]

SalI, EcoRI

LipaDecFW, LipaDecBW

pCWA-aDec

pLp_0373sOFAcwa2

[36]

SalI, MluI

CwaaDecFW, CwaaDecBW

pLysM-aDec

pLp_3014Inv

[31]

SalI, EcoRI

LysMdecFW, LysMdecBW