Table 1 Bacterial strains and plasmids used in this study
Designation | Strains | Characteristic | References |
|---|---|---|---|
| Â | Lb. Plantarum WCFS1 | Host strain | [34] |
| Â | E. coli TOP10 | Host strain | Invitrogen |
| Â | Lp-WT | WCFS1 with pEV | This study |
| Â | Lp-WT/pValac-GFP | WCFS1 with pEV and pValac-GFP | This study |
aDec strains | Lp-Lip-aDec | WCFS1 with pLip-aDec | This study |
Lp-CWA-aDec | WCFS1 with pCWA-aDec | This study | |
Lp-LysM-aDec | WCFS1 with pLysM-aDec | This study | |
Lp-Lip-aDec/pValac-GFP | WCFS1 with pLip-aDec and pValac-GFP | This study | |
Lp-CWA-aDec/pValac-GFP | WCFS1 with pCWA-aDec and pValac-GFP | This study | |
Lp-LysM-aDec/pValac-GFP | WCFS1 with pLysM-aDec and pValac-GFP | This study |
Designation | Plasmids | Characteristic | References |
|---|---|---|---|
| Â | pEV | Control plasmid | [31] |
aDec plasmids | pLip-aDec | Plasmid expressing aDec with lipoanchor | This study |
pCWA-aDec | Plasmid expressing aDec with LPXTG anchor | This study | |
pLysM-aDec | Plasmid expressing aDec with LysM anchor | This study | |
| Â | pValac-GFP | Plasmid expressing GFP under CMV promoter | [32] |