Figure 3From: Surface display of an anti-DEC-205 single chain Fv fragment in Lactobacillus plantarum increases internalization and plasmid transfer to dendritic cells in vitro and in vivoEffect of surface expression of aDec on plasmid delivery into DCs. a Monocyte derived hDCs were co-incubated with Lp-WT/pValac-GFP, Lp-Lip-aDec/pValac-GFP, Lp-CWA-aDec/pValac-GFP or Lp-LysM-aDec/pValac-GFP with or without addition of a free anti-human DEC205 antibody. After incubation the percentage of green fluorescent DCs was determined by flow cytometry. b BMDCs were co-incubated with Lp-WT/pValac-GFP, Lp-Lip-aDec/pValac-GFP, Lp-CWA-aDec/pValac-GFP or Lp-LysM-aDec/pValac-GFP. After incubation, the percentage of fluorescent DCs was determined by flow cytometry. Each point represents independent wells and the results are presented as mean ± SEM. The results presented are from one experiment representative of three performed independently. Statistically significant differences are indicated as follows: *p < 0.05; **p < 0.01; ***p < 0.001.Back to article page