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Figure 3 | Microbial Cell Factories

Figure 3

From: Surface display of an anti-DEC-205 single chain Fv fragment in Lactobacillus plantarum increases internalization and plasmid transfer to dendritic cells in vitro and in vivo

Figure 3

Effect of surface expression of aDec on plasmid delivery into DCs. a Monocyte derived hDCs were co-incubated with Lp-WT/pValac-GFP, Lp-Lip-aDec/pValac-GFP, Lp-CWA-aDec/pValac-GFP or Lp-LysM-aDec/pValac-GFP with or without addition of a free anti-human DEC205 antibody. After incubation the percentage of green fluorescent DCs was determined by flow cytometry. b BMDCs were co-incubated with Lp-WT/pValac-GFP, Lp-Lip-aDec/pValac-GFP, Lp-CWA-aDec/pValac-GFP or Lp-LysM-aDec/pValac-GFP. After incubation, the percentage of fluorescent DCs was determined by flow cytometry. Each point represents independent wells and the results are presented as mean ± SEM. The results presented are from one experiment representative of three performed independently. Statistically significant differences are indicated as follows: *p < 0.05; **p < 0.01; ***p < 0.001.

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