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Figure 5 | Microbial Cell Factories

Figure 5

From: Redox engineering by ectopic expression of glutamate dehydrogenase genes links NADPH availability and NADH oxidation with cold growth in Saccharomyces cerevisiae

Figure 5

Exposure to low temperature increases the fermentative/oxidative balance and the need for glycerol synthesis. a Growth of the S0329-derivative yeast strains, wild-type (Control), gpd1 mutant (gpd1), and gpd1 containing in addition a replacement of the native promoter of GPD2 by a mutated promoter of the unregulated TEF1 yeast gene (gpd1 pTEF1-GPD2) was examined as described in the Figure 2. b Time–course graph of total gas produced for 360 min by cell suspensions of the baker’s yeast strain HS13 grown at 30 or 15°C. Cells were collected and the amount of CO2 evolved at 30°C recorded in a Fermograph II apparatus as described in the “Methods” section. A representative experiment is shown.

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