Figure 3From: Redox engineering by ectopic expression of glutamate dehydrogenase genes links NADPH availability and NADH oxidation with cold growth in Saccharomyces cerevisiae Increased activity of the glutamate dehydrogenase (GDH) isoenzymes Gdh1 and Gdh2 reduces the accumulation of ROS at low temperature. Cells of the CEN.PK2-1C strain harboring plasmids, YEplac195 (Control, empty plasmid), YEpGDH1 (GDH1) and YEpGDH2 (GDH2) were cultivated on SCD-Ura at 30°C or 15°C and tested for ROS accumulation by labeling of cells with the fluorescent probe dihydrorhodamine 123 (DHR 123). Data are expressed as the percentage of viable cells showing DHR 123-positive staining by flow cytometry. Values represent the means of at least four independent experiments. The error associated with the points was calculated by using the formula: (1.96 × SD)/√n, where SD is the standard deviation and n is the number of measurements. Values with different letter are significantly different with a p < 0.05.Back to article page