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Figure 3 | Microbial Cell Factories

Figure 3

From: Redox engineering by ectopic expression of glutamate dehydrogenase genes links NADPH availability and NADH oxidation with cold growth in Saccharomyces cerevisiae

Figure 3

Increased activity of the glutamate dehydrogenase (GDH) isoenzymes Gdh1 and Gdh2 reduces the accumulation of ROS at low temperature. Cells of the CEN.PK2-1C strain harboring plasmids, YEplac195 (Control, empty plasmid), YEpGDH1 (GDH1) and YEpGDH2 (GDH2) were cultivated on SCD-Ura at 30°C or 15°C and tested for ROS accumulation by labeling of cells with the fluorescent probe dihydrorhodamine 123 (DHR 123). Data are expressed as the percentage of viable cells showing DHR 123-positive staining by flow cytometry. Values represent the means of at least four independent experiments. The error associated with the points was calculated by using the formula: (1.96 × SD)/√n, where SD is the standard deviation and n is the number of measurements. Values with different letter are significantly different with a p < 0.05.

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