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Figure 2 | Microbial Cell Factories

Figure 2

From: Redox engineering by ectopic expression of glutamate dehydrogenase genes links NADPH availability and NADH oxidation with cold growth in Saccharomyces cerevisiae

Figure 2

Ectopic expression of GDH1 and GDH2 affects the growth of S. cerevisiae strains at low temperature. a YEplac195 (URA3; Control, empty plasmid), YEpGDH1 (GDH1) and YEpGDH2 (GDH2) transformants of the CEN.PK2-1C and W303-1A wild-type strains were assayed for growth at low temperature. Cultures were incubated on SCD-Ura at 30°C until the exponential phase and were adjusted to OD600 = 1.0. Then, serial dilutions (1–10−3) of the cultures were spotted (3 μl) onto SCD-Ura agar medium and incubated at 30°C for 2 days or at 15°C for 10 days. b Growth of the CEN.PK2-1C transformants was examined on SCD-Ura medium supplemented with 200 μM of tryptophan (+Trp), 32 μM of nicotinic acid (+NA) or the same amount of both (+Trp +NA). In all cases, a representative experiment is shown.

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Microbial Cell Factories

ISSN: 1475-2859

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