Figure 1
From: CrEdit: CRISPR mediated multi-loci gene integration in Saccharomyces cerevisiae
Schematic overview of the CrEdit system. a Replicative vectors expressing gRNAs and cas9. b Targeted DSBs mediated by Cas9 endonuclease activity facilitate the integration of linearized donor plasmids by homologous recombination. Donor plasmids harbor the desired integration sequences flanked by homology arms. Selected intergenic safe harbor sites can be used for simultaneous integration of multiple genomic expression cassettes for pathway engineering.