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Table 3 Plasmids used in this study

From: Establishment of markerless gene deletion tools in thermophilic Bacillus smithii and construction of multiple mutant strains

Plasmid

Description

Reference/origin

pNW33n

E. coli-Bacillus shuttle vector, cloning vector, CmR

BGSC

pWUR732

ldhL-KO vector for ET138: pNW33n + ldhL-flanks

This study

pWUR733

ldhL-KO vector for DSM 4216: pNW33n + ldhL-flanks

This study

pWUR734

pNW33n + B. coagulans P pta -lacZ

[14]; this study

pWUR735

sigF-KO vector for ET138: pWUR734 + ET 138 sigF-flanks

This study

pWUR736

ldhL-restoration vector for ET138: pNW33n + ldhL-gene from ET 138 under its native promoter (525 bp us of ldhL)

This study

pWUR737

pdhA-KO vector for ET138: pWUR734 + ET 138 pdhA -flanks

This study

  1. Cm R chloramphenicol resistance gene, KO knockout, BGSC Bacillus Genetic Stock Centre, USA, us upstream, bp base pairs.