Table 1 Gene-Ontology (GO) database search in EcoCyc of the knockout mutants selected in based on the statistically significant results of the parameters evaluated shown in Figure 3

Fermentation

ldhA//“frdB”//“frmA”//“frdA”//“frdC”

Generation of precursor metabolites and energy

frdB//“tdcE”//“frdA”//“frdC”//“glgC”//“ldhA”//“frmA”//“fumA”

Energy derivation by oxidation of organic compounds

frdB//“tdcE”//“frdA”//“frdC”//“glgC”//“ldhA”//“frmA”

Aspartate family amino acid catabolic process

sucB//“tdcB”//“tdcE”

Bacterial-type flagellum assembly

frdA//“frdB”//“frdC”

Ethanol metabolic process

ldhA//“frmA”

Oxidation–reduction process

gnd//“rpiA”//“frdB”//“tdcE”//“frdA”//“frdC”//“glgC”//“ldhA”//“frmA”

Single-organism metabolic process

rpiA//“gnd”//“deoB”//“gadB”//“tdcB”//“frdB”//“tdcE”//“frdA”//“frdC”//“glgC”//“frmA”//“cpsG”//“ldhA”//“sucB”//“nanE”

Single-organism catabolic process

ldhA//“gnd”//“rpiA”//“deoB”//“tdcB”//“sucB”//“tdcE”//“nanE”//“frmA”

Tricarboxylic acid cycle

fumA//“frdB”//“sucB”

l-threonine catabolic process to propionate

tdcB//“tdcE”

Anaerobic respiration

frdB//“tdcE”//“frdA”//“frdC”

Organic substance catabolic process

frmA//“tdcB”//“sucB”//“tdcE”//“ldhA”//“gnd”//“rpiA”//“nanE”//“deoB”

Glucose catabolic process

ldhA//“gnd”//“rpiA”

Cell motility

frdA//“frdB”//“frdC”

Carbohydrate metabolic process

rpiA//“gnd”//“ldhA”//“glgC”//“cpsG”//“nanE”//“tdcE”

Small molecule metabolic process

frmA//“cpsG”//“deoB”//“gnd”//“rpiA”//“ldhA”//“gadB”//“tdcB”//“sucB”//“tdcE”//“nanE”

Cellular metabolic process

deoB//“frdB”//“tdcE”//“frdA”//“frdC”//“frmA”//“gnd”//“rpiA”//“cpsG”//“glgC”//“ldhA”//“gadB”//“tdcB”//“nanE”//“sucB”//“fumA”

Cellular respiration

frdB//“tdcE”//“frdA”//“frdC”