Table 1 Strains used in this study
Strains | Genotype and/or relevant characteristic(s) | Source |
|---|---|---|
E. coli DH5α | F−∆lacU169(Ø80d lacZ∆M15) supE44 hsdR17 recA1 gyrA96 endA1 thi-1 relA1 | Invitrogen |
B. licheniformis CICC 10181 | Wild-type B. licheniformis, amyl gene | CICC |
G. stearothermophilus ATCC 31195 | Wild-type G. stearothermophilus, amys gene | ATCC |
B. subtilis 1A751 | eglS∆102 bglT/bglS∆EV aprE nprE his | BGSC |
1AXBG | 1A751 with integration of pDDXBG (amyE::P xylA -bgaB); Cmr | This work |
1AXGB | 1A751 with integration of pDDXGB (amyE::P grac -bgaB); Cmr | This work |
1A01 | 1A751 with integration of pDD01 (amyE::P xylA -ffh); Cmr | This work |
1A02 | 1A751 with integration of pDD02 (amyE::P xylA -hbs); Cmr | This work |
1A03 | 1A751 with integration of pDD03 (amyE::P xylA -scr); Cmr | This work |
1A04 | 1A751 with integration of pDD04 (amyE::P xylA -SRP); Cmr | This work |
1A05 | 1A751 with integration of pDD05 (amyE::P xylA -ftsy); Cmr | This work |
1A06 | 1A751 with integration of pDD06 (amyE::P xylA -csaA); Cmr | This work |
1A07 | 1A751 with integration of pDD07 (amyE::P xylA -secA); Cmr | This work |
1A08 | 1A751 with integration of pDD08 (amyE::P xylA -secY); Cmr | This work |
1A09 | 1A751 with integration of pDD09 (amyE::P xylA -secE); Cmr | This work |
1A10 | 1A751 with integration of pDD10 (amyE::P xylA -secG); Cmr | This work |
1A11 | 1A751 with integration of pDD11 (amyE::P xylA -secYEG); Cmr | This work |
1A12 | 1A751 with integration of pDD12 (amyE::P xylA -secDF); Cmr | This work |
1A13 | 1A751 with integration of pDD13 (amyE::P xylA -yrbF); Cmr | This work |
1A14 | 1A751 with integration of pDD14 (amyE::P xylA -spoIIIJ); Cmr | This work |
1A15 | 1A751 with integration of pDD15 (amyE::P xylA -yqjG); Cmr | This work |
1A16 | 1A751 with integration of pDD16 (amyE::P xylA -sipT); Cmr | This work |
1A17 | 1A751 with integration of pDD17 (amyE::P xylA -sipS); Cmr | This work |
1A18 | 1A751 with integration of pDD18 (amyE::P xylA -sipU); Cmr | This work |
1A19 | 1A751 with integration of pDD19 (amyE::P xylA -sipV); Cmr | This work |
1A20 | 1A751 with integration of pDD20 (amyE::P xylA -sipW); Cmr | This work |
1A21 | 1A751 with integration of pDD21 (amyE::P xylA -groESL operon); Cmr | This work |
1A22 | 1A751 with integration of pDD22 (amyE::P xylA -partial dnaK operon); Cmr | This work |
1A23 | 1A751 with integration of pDD23 (amyE::P xylA -prsA); Cmr | This work |
1A231 | 1A751 with integration of pDD231 (amyE::P xylA -prsA, P grac -SRP); Cmr | This work |
1A232 | 1A751 with integration of pDD232 (amyE::P xylA -prsA, P grac -ftsy); Cmr | This work |
1A233 | 1A751 with integration of pDD233 (amyE::P xylA -prsA, P grac -secA); Cmr | This work |
1A234 | 1A751 with integration of pDD234 (amyE::P xylA -prsA, P grac -secYEG); Cmr | This work |
1A235 | 1A751 with integration of pDD235 (amyE::P xylA -prsA, P grac -secDF); Cmr | This work |
1A236 | 1A751 with integration of pDD236 (amyE::P xylA -prsA, P grac -groESL operon); Cmr | This work |
1A237 | 1A751 with integration of pDD237 (amyE::P xylA -prsA, P grac -partial dnaK opeon); Cmr | This work |
1A238 | 1A751 with integration of pDD238 (amyE::P xylA -prsA, P grac -sipT); Cmr | This work |
1A239 | 1A751 with integration of pDD239 (amyE::P xylA -prsA, P grac -sipS); Cmr | This work |