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Figure 5 | Microbial Cell Factories

Figure 5

From: Combinatorial Sec pathway analysis for improved heterologous protein secretion in Bacillus subtilis: identification of bottlenecks by systematic gene overexpression

Figure 5

Characterization of engineered and parental strains. B. subtilis 1A751 (pMA5L) (parental strain), 1A237 (pMA5L) (engineered strain), 1A751 (pMA5S) (parental strain) and 1A237 (pMA5S) (engineered strain) were cultivated for 96 h at 37°C in 30 mL SR medium supplemented with 4% xylose (w/v). a Growth of the strains. b Analysis of α-amylase (AmyL and AmyS) activity. c Analysis of productivity. d SDS-PAGE analysis of α-amylase (AmyL and AmyS) at 72 h.

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Microbial Cell Factories

ISSN: 1475-2859

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