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Figure 3 | Microbial Cell Factories

Figure 3

From: Combinatorial Sec pathway analysis for improved heterologous protein secretion in Bacillus subtilis: identification of bottlenecks by systematic gene overexpression

Figure 3

Optimization of PrsA overexpression level in B. subtilis. 1A23 (pMA5L) and 1A23 (pMA5S) were grown in SR medium supplemented with xylose of different concentrations [0, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, 4.0, 4.5 and 5.0 % (w/v)]. a Analysis of α-amylase (AmyL and AmyS) activity in the supernatant at incubation of 72 h. The α-amylase activity was converted to percent increase referring to 2.0% xylose. b SDS-PAGE analysis of α-amylase (AmyL and AmyS) distribution in culture supernatants.

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Microbial Cell Factories

ISSN: 1475-2859

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