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Figure 2 | Microbial Cell Factories

Figure 2

From: Combinatorial Sec pathway analysis for improved heterologous protein secretion in Bacillus subtilis: identification of bottlenecks by systematic gene overexpression

Figure 2

Overexpression of single component involved in Sec pathway in B. subtilis. a The transcriptional levels of 23 genes or gene operons in 23 corresponding recombinant strains, respectively. Column Control represents the transcriptional levels of the 23 genes or gene operons in 1A751 (all regarded as 100%). b Effect of overexpression of single gene or gene operon on AmyL secretion. Column Control is the α-amylase activity of 1A751 (pMA5L), regarded as 100%. c Effect of overexpression of single gene or gene operon on AmyS secretion. Column Control is the α-amylase activity of 1A751 (pMA5S), served as 100%. The samples were collected at incubation of 72 h in 250 mL shake-flask and centrifuged at 10,000×g and 4°C for 10 min. After extraction, the supernatant was used for analysis of α-amylase activity. The α-amylase activity was converted to percent increase referring to the respective control strains. Data represent the mean of three parallel experiments, and error bars represent standard error. Specifically, the genes or gene operons ffh, hbs, scr, SRP, ftsy, csaA, secA, secY, secE, secG, secYEG, secDF, yrbF, spoIIIJ, yqjG, sipT, sipS, sipU, sipV, sipW, groESL operon, partial dnaK operon and prsA in a, b and c corresponded to strains 1A01, 1A02, 1A03, 1A04, 1A05, 1A06, 1A07, 1A08, 1A09, 1A10, 1A11, 1A12, 1A13, 1A14, 1A15, 1A16, 1A17, 1A18, 1A19, 1A20, 1A21, 1A22 and 1A23, respectively.

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