Table 4 PCR primers that designed for the upstream and downstream DNA sequence amplification of PCR amplified pln genes harboured in Lactobacillus plantarum I-UL4
Primer designation | Primer sequence (5′–3′) |
|---|---|
brnQ1-napA1 | F: ATGCTCTTTGGGATGTTTTT |
R: ACGATGAAATAGCGGTGAGG | |
napA1-L | F: GTGGGCTTGAGTGGTGCTAT |
R: TACTTTACGGAGCGGCATCT | |
R-plnD | F: AGCAGCCCCATCACTAATC |
R: AACATCTTTGCGCTGACTATT | |
plnD-I | F: TGAGGACAAACAGACTGGAC |
R: AGTCTGCCTTTGAGCCTAGC | |
plnI-EF | F: CGTTAATGGGTGATTGAGTTG |
R: CACGGATATAGTTCAAGCCATC | |
plnEF-G | F: CGGTCACGCAAAACTAGAAAT |
R: TCAATCACCGCTTGTAAGAAA | |
plnG-H | F: TTATTGGCGGTTTTAGGTCA |
R: CGCGCACCTTCAACTAAATA | |
plwG1 | F: CGGAATGTGGACTTTGTTGT |
R: TGCTGGCTTCCATTATTTCA | |
brnQ1-walk | F: CCAAGGGGGTCTTTGTAGGT |
R: CCAAAGTCGCACAAGTCAGTA | |
plnU-helicase | F: ATTTTGAGATGCCAGTCCTGTT |
R: TGGTCGCATACGATGTCTCC | |
Plw | F: CGCTTGCCAATGAACAAATA |
R: CGCCAATCGGGAATTTATCA | |
plwGTSP | F1: AGATGAGGCGACTAGCAGTGT |
F2: GGTGAAAATTTGAGAAAGGACAG | |
F3: TGTAGCACATCGACTATCAACCA |