Molecular characterisation of new organisation of plnEF and plw loci of bacteriocin genes harbour concomitantly in Lactobacillus plantarum I-UL4

Tai, Hui Fong; Foo, Hooi Ling; Abdul Rahim, Raha; Loh, Teck Chewn; Abdullah, Mohd. Puad; Yoshinobu, Kimura

doi:10.1186/s12934-015-0280-y

Table 3 PCR primers that designed for the detection of pln genes haboured in Lactobacillus plantarum I-UL4

From: Molecular characterisation of new organisation of plnEF and plw loci of bacteriocin genes harbour concomitantly in Lactobacillus plantarum I-UL4

Target gene	Function	Primer sequence (5′–3′)	Size (bp)	References
brnQ1	Amino acid transport protein	F: ATGCTCTTTGGGATGTTTTT	1,068	[23]
brnQ1	Amino acid transport protein	R: ACGATGAAATAGCGGTGAGG	1,068	[23]
napA1	Na-⁺/H⁺ antiporter	F: AAGTATTTACGCCCTGCCATTA	798	[23]
napA1	Na-⁺/H⁺ antiporter	R: TTAAACCCACACTGACGAAGAA	798	[23]
plnJ	Prebacteriocin	F: TAACGACGGATTGCTCTG	475	[22]
plnJ	Prebacteriocin	R: AATCAAGGAATTATCACATTAGTC	475	[22]
plnK	Prebacteriocin	F: CTGTAAGCATTGCTAACCAATC	246	[22]
plnK	Prebacteriocin	R: ACTGCTGACGCTGAAAAG	246	[22]
plnL	Putative immunity protein	F: TAGATGCCGCTCCGTAAAGT	442	[22]
plnL	Putative immunity protein	R: CGTTACCCTCGCCAAAGTG	442	[22]
plnM	Unknown function	F: TGCTTGAAAGAATTACAGGATT	171	[22]
plnM	Unknown function	R: CAAACGCAACCATCAAAATA	171	[22]
plnN	Prebacteriocin	F: ATTGCCGGGTTAGGTATCG	146	[22]
plnN	Prebacteriocin	R: CCTAAACCATGCCATGCAC	146	[22]
plnO	Glycosyl transferase group 2 family	F: CGGAGACCCTTTATTATTTTG	580	[22]
plnO	Glycosyl transferase group 2 family	R: TCTTCGGACCCCTCTGATT	580	[22]
plnP	Protease CAAX family	F: TCCGAAAAGTATGGACAAATGA	437	[22]
plnP	Protease CAAX family	R: AAAGTTCCCCAAAGCAGACC	437	[22]
plnA	Induction factor	F: CAAATTAAAGGTATGAAGCAACT	113	[22]
plnA	Induction factor	R: TTCTTTACCTGTTTAATTGCAG	113	[22]
plnB	Histidine kinase	F: CTGGCTTGTCGGAGTATGGT	531	[22]
plnB	Histidine kinase	R: CGTCATTTAGGCTTGCTCTG	531	[22]
plnC	Response regulator	F: GGCGACAGGAGATTTACAAGA	437	[22]
plnC	Response regulator	R: CCACTTTATTTTTGGCAGTCAG	437	[22]
plnD	Response regulator	F: TGAGGACAAACAGACTGGAC	415	[22]
plnD	Response regulator	R: GCATCGGAAAAATTGCGGATAC	415	[22]
plnEF	Prebacteriocin	F: GGCATAGTTAAAATTCCCCCC	428	[22]
plnEF	Prebacteriocin	R: CAGGTTGCCGCAAAAAAAG	428	[22]
plnI	Immunity	F: CGTTAATGGGTGATTGAGTTG	424	[22]
plnI	Immunity	R: AGTCTGCCTTTGAGCCTAGC	424	[22]
plnG	ABC transporter	F : TGCGGTTATCAGTATGTCAAAG	454	[22]
plnG	ABC transporter	R: CCTCGAAACAATTTCCCCC	454	[22]
plnH	Accessory protein	F: AGTTTTACGGGATTCGGTTT	986	[22]
plnH	Accessory protein	R: CTTTGCACCACGGTAATTGT	986	[22]
plw	Prebacteriocin	F: AGTCGTCGTAAGAATGCTATTG	389	[19]
plw	Prebacteriocin	R: TCACACGAAATATTCCA	389	[19]
plwG	ABC transporter	F: GGTGTACTGGACTTAGGCATGG	1,034	[19]
plwG	ABC transporter	R: CGCTCTCGCAATCGTTATTC	1,034	[19]
plnC8	Prebacteriocin	F: GGTCTGCGTATAAGCATCGC	207	[27]
plnC8	Prebacteriocin	R: AAATTGAACATATGGGTGCTTTAAATTCC	207	[27]
plNC8HK	Histidine kinase	F: AGCGGCAGTTATGGTAGGAC	790	[27]
plNC8HK	Histidine kinase	R: AATCCCTTTAGTTTGGGCATC	790	[27]
plaC	ABC transporter	F: GGCGTCTTTCTTGCTTTTG	301	[21]
plaC	ABC transporter	R: ACCCGTTGTTCCCATAGTC	301	[21]
plaD	Accessory protein	F: TGGACTCAAAAATGGCACAA	950	[21]
plaD	Accessory protein	R: GGAACCACAACTAACGAGCA	950	[21]
plS	Prebacteriocin	F: GCCTTACCAGCGTAATGCCC	320	[20]
plS	Prebacteriocin	R: CTGGTGATGCAATCGTTAGTTT	320	[20]
16S rDNA	Positive control	PLANT1 : ATCATGATTTACATTTGAGTG	996	[62]
16S rDNA	Positive control	LOWLAC: CGACGACCATGAACCACCTGT	996	[62]

F forward primer, R reverse primer.

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ISSN: 1475-2859

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