Table 2 Characteristics of the predicted ORFs encoded by UL4-plnEF locus amplified from Lactobacillus plantarum I-UL4
Predicted ORFs | Orientation (+ or −) | Nucleotide coordinates | Gene and peptide length (bp: aa) | 15 bp upstream of the start codon (5′–3′) | Homologous gene and function | Re-designated as |
|---|---|---|---|---|---|---|
ef1 | + | 829–2,205 | 1,377: 458 | GGAGGAGAGACGACT | brnQ1: amino acid transporter | brnQ1 |
ef2 | + | 2,238–3,434 | 1,197: 398 | TAAGACTTTTGATGG | napA1: Na+/H+ antiporter | napA1 |
ef3 | + | 3,809–3,982 | 174: 57 | GAAAAGGTGATTAAA | orf3: putative prebacteriocin | orf3 |
ef4 | + | 3,998–4,177 | 180: 59 | AAAGAAGTGGTAAAA | orf4: putative prebacteriocin | orf4 |
ef5 | + | 4,283–4,525 | 243: 80 | TTGTTTGTTCTTTTA | orf5: putative immunity protein | orf5 |
ef6 | – | 4,970–5,083 | 114:37 | GTAAGGCACACGTTA | plnR: unknown | plnR |
ef7 | – | 5,108–5,776 | 669: 222 | CTCGGGGGATTATAA | plnL: putative immunity protein | plnL |
ef8 | + | 6,369–6,536 | 168: 55 | GGAGGGGTTATTATT | Putative induction factor | UL4IF |
ef9 | + | 6,554–7,894 | 1,341: 446 | TAGGTGGTGTTCCAC | HK: histidine Protein Kinase | UL4HK |
ef10 | + | 7,895–8,638 | 744: 247 | TTGGAGGAAGAATGA | plnD: response regulator | plnD |
ef11 | – | 8,932–9,705 | 774: 257 | GGGGGAATTTTAACT | plnI: immunity protein | plnI |
ef12 | – | 9,784–9,963 | 180: 59 | GGGAGATCAACAATT | plnF: plantaricin EF precursor | plnF |
ef13 | – | 9,988–10,158 | 171: 56 | CAAGGGGGATTATTT | plnE: plantaricin EF precursor | plnE |
ef14 | + | 10,424–12,574 | 2,151: 716 | GAGGGGAGTACAAGT | plnG: ABC transporter | plnG |
ef15 | + | 12,591–13,967 | 1,377: 458 | GGGGGAAACTGAATA | plnH: accessory protein | plnH |
ef16 | + | 14,057–14,746 | 690: 229 | CGAAAGAGGTAAGTA | plnT: unknown | plnT |
ef17 | + | 14,814–15,482 | 669: 222 | CTTGGGAGGCTTGGT | plnU: unknown | plnU |
ef18 | + | 15,569–16,249 | 681: 226 | TGGATGTGAAGGAGC | plnV: unknown | plnV |
ef19 | + | 16,343–17,029 | 687: 228 | GATGGAGTGGATGAA | plnW: unknown | plnW |
ef20 | + | 17,167–17,370 | 204: 67 | AGGAGTTTGGTAAGT | orfZ1: unknown | UL4orfZ1 |
ef21 | – | 17,465–>17,588 | >124:>40 | ND | DHelicase: DNA helicase | DHelicase |