Table 2 Microbial strains and plasmids used in this study
Description | Source or reference | |
|---|---|---|
Strains | ||
JCM9042 | Wild-type strain of P. rhodozyma | Institute of Physical and Chemical Research (RIKEN), Japan |
MK19 | Generated by NTG and Co60 mutagenesis from JCM9042 | Miao et al. [11] |
CSR19 | CrtS-overexpressing strain (with Rbs of P. rhodozyma crtS gene) | Present study |
CS19 | CrtS-overexpressing strain (with Rbs of plasmid pGBKT7) | Present study |
E. coli DH5α | EndA1 hsdR17 [r-m+] supE44 thi-1 recA1 gyrA [NalR]relA1△[lacZYA-argF]U169deoR [Φ80△]M15] | Green et al. [26] |
Plasmids | ||
pGBKT7 | Expression vector, ADH1 promoter, Kmr | Donated by Professor Huiqiang Lou |
pGBKT7-crtSr | pGBKT7 derivative containing crtSr gene (with Rbs of P. rhodozyma crtS gene) | Present study |
pGBKT7-crtS | pGBKT7 derivative containing crtS gene (with Rbs of plasmid pGBKT7) | Present study |
pMDTM18-T | Cloning vector for gene sequencing, Ampr | Takara Co., Japan |