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Table 2 Strains used in this study, all strains are derived from BY4741 (MATa his3∆0 leu2∆0 met15∆0 ura3∆0), with the additional TRP1 gene knocked out to allow for selection with pCM plasmids

From: Expression of RCK2 MAPKAP (MAPK-activated protein kinase) rescues yeast cells sensitivity to osmotic stress

Strains used in this study

Integrative plasmida

BY4741Δtrp1 (MATa his3∆0 leu2∆0 met15∆0 ura3∆0 trp1Δ0)

–

BY4741Δtrp1Δrck2

–

BY4741Δtrp1Δrck2

pCM161

BY4741Δtrp1Δrck2

pCM161 (RCK2)

  1. aIntegrative plasmids were constructed as indicated in “Plasmid construction”.
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Microbial Cell Factories

ISSN: 1475-2859

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