Table 1 Carbon balance for batch cultivation of Δrck2pCM161(RCK2) and Δrck2pCM161(RCK2) strains during a 15% glucose fermentation
Substrate and products | Phase I (0–21 h) | Phase II (21–40 h) | ||||
|---|---|---|---|---|---|---|
Amount (mM) | Carbon (mM) | Carbon (%) | Amount (mM) | Carbon (mM) | Carbon (%) | |
Δrck2pCM161 | ||||||
Glucose | 426.1 | 2556.6 | 100.0 | 354.1 | 2124.6 | 100.0 |
Ethanol | 481.1 | 962.2 | 37.6 | 781.3 | 1562.6 | 73.5 |
Glycerol | 2.9 | 8.7 | 0.3 | 0.5 | 1.5 | 0.1 |
Acetic acid | 1.0 | 2.0 | 0.1 | 0.6 | 1.2 | 0.1 |
Trehalose | 1.2 | 7.2 | 0.3 | 0.0 | 0.0 | 0.0 |
Glycogen | 1.1 | 6.6 | 0.3 | 0.4 | 2.4 | 0.1 |
CO2 | 481.1 | 481.1 | 18.8 | 781.3 | 781.3 | 36.8 |
Carbon recovery | 57.6 | 110.6 | ||||
Δrck2pCM161(RCK2) | ||||||
Glucose | 559.4 | 3356.4 | 100.0 | 228.4 | 1370.4 | 100.0 |
Ethanol | 831.1 | 1662.2 | 49.5 | 411.6 | 823.2 | 60.1 |
Glycerol | 1.9 | 5.7 | 0.2 | 1.5 | 4.5 | 0.3 |
Acetic acid | 1.2 | 2.4 | 0.1 | 0.5 | 1.0 | 0.1 |
Trehalose | 1.0 | 6.0 | 0.2 | 0.4 | 2.4 | 0.2 |
Glycogen | 1.1 | 6.6 | 0.2 | 0.4 | 2.4 | 0.2 |
CO2 | 831.1 | 831.1 | 24.8 | 411.6 | 411.6 | 30.0 |
Carbon recovery | 74.9 | 90.9 | ||||