Table 2 PCR primers used in this study
From: Determination of key enzymes for threonine synthesis through in vitro metabolic pathway analysis
Enzyme name | Prime name | Primer sequence (5′–3′) |
|---|---|---|
ThrA | thrA_F1 | GGGAATTCCATATG ATGCGAGTGTTGAAGTTCGGCGGTA |
thrA_R1 | CCGGAATTCTCAGACTCCTAACTTCCATGAGAGG | |
LysC | lysC_F1 | GGGAATTCCATATG ATGTCTGAAATTGTTGTCTCCAAAT |
lysC_R1 | CCGGAATTCTTACTCAAACAAATTACTATGCAGT | |
ASD | asd_F1 | CTAGCTAGCATGAAAAATGTTG GTT TTATCGGCT |
asd_R1 | CCGGAATTCTTACGCCAGTTGACGAAGCATCCGA | |
ThrB | thrB_F1 | GGGAATTCCATATGATGGTTAAAGTTTATGCCCCGGCTT |
thrB_R1 | CCG GAATTCTTAGTTTTCCAGTACTCGTGCGCCC | |
ThrC | thrC_F1 | GGGAATTCCATATGATGAAACTCTACAATCTGAAAGATC |
thrC_R1 | CGCGGATCCTTACTGATGATTCATCATCAATTTA | |
LysC | lysC_F2 | CCG GAATTCATG ATGTCTGAAATTGTTGTCTCCAAAT |
lysC_R2 | CGC GGATCCTTACTCAAACAAAT TACTATGC AGT |