Figure 8
From: Determination of key enzymes for threonine synthesis through in vitro metabolic pathway analysis
The pathway flux (J) in the vitro system when enzyme concentration was increased. a The pathway flux in the system caused by crude enzyme extract without addition of purified enzyme. b The pathway flux when purified LysC was added to the crude enzyme extract. The good linear relationships between threonine concentration and reaction time indicate that the in vitro pathway was at pseudo-steady state during that time period. Therefore the threonine production rates calculated from the linear relationship can represent the pathway fluxes. Three replicates of this experiment.