SEC-MALS analysis of purified rHtrA proteins. SEC-MALS analysis of (a) purified rHtrA1 (40 kDa) and (b) rHtrA2 (39 kDa), proteins is shown. rHtrA proteins at 1 mg/mL (small dash line), 2 mg/mL (large dash line) and 4 mg/mL (solid line) were loaded on a 15 mL KW-803 column (Shodex). Absorbance at 280 nm (on the left) and molar mass (on the right) are plotted as a function of the elution volume. Results obtained with rHtrA1 were confirmed using a Superdex 200 increase 10/300 GL column (GE, data not shown). RU relative unit.