Table 2 Quantification of intein-mediated protein production
From: Formation of active inclusion bodies induced by hydrophobic self-assembling peptide GFIL8
Product protein (molecular weight) | Aggregatesa (μg/mg wet cell pellet) | Quantity of purified proteina (μg/mg wet cell pellet) | Specific activity (U/mg enzyme) | Cleavage efficiencyb (%) | Percent recoveryc (mass) |
|---|---|---|---|---|---|
For GFIL8 fusion | |||||
LipA (21 kDa) | 27.1 ± 0.7 | 7.5 ± 0.7 | 34.6 ± 2.2 | 64.0 ± 2.7 | 58.6 ± 4.8 |
AMA (49 kDa) | 21.4 ± 1.8 | 3.0 ± 0.3 | 1656.5 ± 48.9 | 64.5 ± 2.7 | 20.6 ± 0.9 |
For GFIL16 fusion | |||||
LipA | 4.7 ± 0.4 | 33.8 ± 0.5 | 49.0 ± 4.3 | ||
AMA | 4.3 ± 0.7 | 1567.7 ± 43.3 | 41.1 ± 5.5 | ||