From: Formation of active inclusion bodies induced by hydrophobic self-assembling peptide GFIL8
Enzymes | Activity (U/ml)a | Percent of activity in insoluble fractions (PDE)b | Percent of amount in insoluble fractionsc | Specific activity (U/mg enzyme)d | Specific activity to the native enzyme in the studied phase (%) | |
---|---|---|---|---|---|---|
Soluble fractions | Insoluble fractions | |||||
LipA-native | 11.3 ± 1.7 | 1.3 ± 0.1 | 10.3 | 20.3 | 28.9 ± 4.4 | 100 |
LipA-GFIL8 | 1.4 ± 0.1 | 7.9 ± 1.0 | 84.9 | 88.6 | 12.5 ± 1.7 | 43.3 |
AMA-native | 952.8 ± 34.0 | 22.5 ± 3.7 | 2.3 | 21.2 | 1591.7 ± 56.8 | 100 |
AMA-GFIL8 | 167.7 ± 2.8 | 332.5 ± 9.8 | 66.5 | 93.2 | 865.5 ± 91.4 | 54.4 |