Figure 2
From: Formation of active inclusion bodies induced by hydrophobic self-assembling peptide GFIL8
Fusion proteins expression and intein-mediated cleavage. a LipA-native and LipA-GFIL8. b AMA-native and AMA-GFIL8. c LipA-I-GFIL8. d AMA-I-GFIL8. e LipA-I-GFIL16. f AMA-I-GFIL16. For a–f lane 1 soluble fraction of cell lysate; lane 2 insoluble fraction of cell lysate; lane 3 insoluble fraction of cleaved fusion protein; lane 4 soluble fraction of cleaved fusion protein. Lane I, II, III and IV, bovine serum albumin (BSA) standards, at 6, 3, 1.5 and 0.75 μg/lane, respectively.