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Table 2 Parameter profiles in 1.5-mL-scale microfuge tube succinate fermentation reactions

From: Impact of an energy-conserving strategy on succinate production under weak acidic and anaerobic conditions in Enterobacter aerogenes

Strain

End-pH

Biomassa, g[DCW]/L

Consumed glucose, g/L

End-product, g/L

Succinate Yieldb, % (g/g)

Pyruvate

Lactate

Malate

Succinate

Formate

Acetate

ES04c

5.8 ± 0.1

5.2 ± 0.3d

8.1 ± 0.6

0.8 ± 0.1

<0.1

<0.1

1.6 ± 0.1

0.6 ± 0.1

1.4 ± 0.1

19.7

ES04/PCK

5.6 ± 0.1

5.7 ± 0.3

10.4 ± 0.6

0.4 ± 0.1

<0.1

0.4 ± 0.1

6.4 ± 0.1

0.3 ± 0.1

0.8 ± 0.1

61.5

ES05

5.8 ± 0.1

5.2 ± 0.3

7.4 ± 0.4

1.1 ± 0.2

<0.1

<0.1

1.6 ± 0.1

0.7 ± 0.1

0.9 ± 0.1

21.6

ES05/PCK

5.6 ± 0.1

5.7 ± 0.3

11.0 ± 0.6

0.5 ± 0.1

<0.1

0.4 ± 0.1

6.8 ± 0.1

0.3 ± 0.1

0.6 ± 0.1

61.8

ES06

5.5 ± 0.1

5.8 ± 0.3

12.0 ± 0.4

1.0 ± 0.1

<0.1

0.5 ± 0.1

7.6 ± 0.4

0.3 ± 0.1

0.6 ± 0.1

63.3

ES07

5.5 ± 0.1

5.9 ± 0.3

14.2 ± 0.8

1.0 ± 0.1

<0.1

0.2 ± 0.1

9.1 ± 0.3

0.4 ± 0.1

0.5 ± 0.1

64.1

ES08

5.5 ± 0.1

5.8 ± 0.3

14.5 ± 0.8

1.0 ± 0.1

<0.1

0.2 ± 0.1

9.2 ± 0.3

0.2 ± 0.1

0.5 ± 0.1

63.4

ES08ΔptsG

5.7 ± 0.1

5.4 ± 0.4

11.5 ± 0.3

0.6 ± 0.1

<0.1

0.1 ± 0.1

8.8 ± 0.2

0.2 ± 0.1

0.7 ± 0.1

76.5

ES08ΔptsI

5.9 ± 0.1

5.3 ± 0.3

6.2 ± 0.8

0.3 ± 0.1

ND

0.1 ± 0.1

5.4 ± 0.3

<0.1

0.3 ± 0.1

87.1

  1. ND not determined.
  2. aInitial biomass was adjusted to ~5.0 g[DCW]/L.
  3. bSuccinate yield is grams of product per grams of consumed glucose expressed as a percentage.
  4. cAll the mutants were cultivated for 24 h.
  5. dData are expressed as mean ± SD of four independent experiments.