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Table 2 Parameter profiles in 1.5-mL-scale microfuge tube succinate fermentation reactions

From: Impact of an energy-conserving strategy on succinate production under weak acidic and anaerobic conditions in Enterobacter aerogenes

Strain End-pH Biomassa, g[DCW]/L Consumed glucose, g/L End-product, g/L Succinate Yieldb, % (g/g)
Pyruvate Lactate Malate Succinate Formate Acetate
ES04c 5.8 ± 0.1 5.2 ± 0.3d 8.1 ± 0.6 0.8 ± 0.1 <0.1 <0.1 1.6 ± 0.1 0.6 ± 0.1 1.4 ± 0.1 19.7
ES04/PCK 5.6 ± 0.1 5.7 ± 0.3 10.4 ± 0.6 0.4 ± 0.1 <0.1 0.4 ± 0.1 6.4 ± 0.1 0.3 ± 0.1 0.8 ± 0.1 61.5
ES05 5.8 ± 0.1 5.2 ± 0.3 7.4 ± 0.4 1.1 ± 0.2 <0.1 <0.1 1.6 ± 0.1 0.7 ± 0.1 0.9 ± 0.1 21.6
ES05/PCK 5.6 ± 0.1 5.7 ± 0.3 11.0 ± 0.6 0.5 ± 0.1 <0.1 0.4 ± 0.1 6.8 ± 0.1 0.3 ± 0.1 0.6 ± 0.1 61.8
ES06 5.5 ± 0.1 5.8 ± 0.3 12.0 ± 0.4 1.0 ± 0.1 <0.1 0.5 ± 0.1 7.6 ± 0.4 0.3 ± 0.1 0.6 ± 0.1 63.3
ES07 5.5 ± 0.1 5.9 ± 0.3 14.2 ± 0.8 1.0 ± 0.1 <0.1 0.2 ± 0.1 9.1 ± 0.3 0.4 ± 0.1 0.5 ± 0.1 64.1
ES08 5.5 ± 0.1 5.8 ± 0.3 14.5 ± 0.8 1.0 ± 0.1 <0.1 0.2 ± 0.1 9.2 ± 0.3 0.2 ± 0.1 0.5 ± 0.1 63.4
ES08ΔptsG 5.7 ± 0.1 5.4 ± 0.4 11.5 ± 0.3 0.6 ± 0.1 <0.1 0.1 ± 0.1 8.8 ± 0.2 0.2 ± 0.1 0.7 ± 0.1 76.5
ES08ΔptsI 5.9 ± 0.1 5.3 ± 0.3 6.2 ± 0.8 0.3 ± 0.1 ND 0.1 ± 0.1 5.4 ± 0.3 <0.1 0.3 ± 0.1 87.1
  1. ND not determined.
  2. aInitial biomass was adjusted to ~5.0 g[DCW]/L.
  3. bSuccinate yield is grams of product per grams of consumed glucose expressed as a percentage.
  4. cAll the mutants were cultivated for 24 h.
  5. dData are expressed as mean ± SD of four independent experiments.