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Table 1 Microbial strains and plasmids used in this study

From: Impact of an energy-conserving strategy on succinate production under weak acidic and anaerobic conditions in Enterobacter aerogenes

Strains and plasmids Description Antibiotic resistance References or sources
Strain
 AJ110637 Isolated wild-type strain (FERM BP-10955) [20]
 ES04 AJ110637 ΔadhE ΔldhA Δpta ΔbudA Km [21]
 ES04/PCK AJ110637 ΔadhE ΔldhA Δpta ΔbudA harboring pSTV28-pck Km, Cm [21]
 ES04/PCK + PYC AJ110637 ΔadhE ΔldhA Δpta ΔbudA harboring pSTV28-pck + pyc Km, Cm [21]
 ES05 AJ110637 ΔadhE ΔldhA Δpta ΔbudA ΔpoxB Km This work
 ES05/PCK AJ110637 ΔadhE ΔldhA Δpta ΔbudA ΔpoxB harboring pSTV28-pck Km, Cm This work
 ES06 AJ110637 ΔadhE ΔldhA Δpta ΔbudA ΔpoxB::P tac -pck (A. succinogenes) Km This work
 ES07 AJ110637 ΔadhE ΔldhA Δpta ΔbudA ΔpoxB::P tac -pck (A. succinogenes) P tac -frdABCD Km This work
 ES08 AJ110637 ΔadhE ΔldhA Δpta ΔbudA ΔpoxB::P tac -pck (A. succinogenes) P tac -frdABCD ΔpflB Km This work
 ES08ΔptsG AJ110637 ΔadhE ΔldhA Δpta ΔbudA ΔpoxB::P tac -pck (A. succinogenes) P tac -frdABCD ΔpflB ΔptsG Km This work
 ES08ΔptsI AJ110637 ΔadhE ΔldhA Δpta ΔbudA ΔpoxB::P tac -pck (A. succinogenes) P tac -frdABCD ΔpflB ΔptsI Km This work
Plasmid
 pRSFRedTER Broad-host-range λ Red-expressing plasmid Cm [44]
 pMW- attL λ-KmR- attR λ Cassette for gene disruption containing kanamycin resistance gene Km [45]
 pRSF-P ara -IX Plasmid for removal of antibiotic resistance genes Cm [21]
 pSTV28 Plasmid vector with a replication origin of pACYC184 Cm TAKARA BIO
 pSTV28-pck Plasmid for expression of pck from A. succinogenes Cm [20]
 pSTV28-pck+pyc Plasmid for co-expression of pck from A. succinogenes and pyc from C. glutamicum Cm [21]