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Table 1 Microbial strains and plasmids used in this study

From: Impact of an energy-conserving strategy on succinate production under weak acidic and anaerobic conditions in Enterobacter aerogenes

Strains and plasmids

Description

Antibiotic resistance

References or sources

Strain

 AJ110637

Isolated wild-type strain (FERM BP-10955)

–

[20]

 ES04

AJ110637 ΔadhE ΔldhA Δpta ΔbudA

Km

[21]

 ES04/PCK

AJ110637 ΔadhE ΔldhA Δpta ΔbudA harboring pSTV28-pck

Km, Cm

[21]

 ES04/PCK + PYC

AJ110637 ΔadhE ΔldhA Δpta ΔbudA harboring pSTV28-pck + pyc

Km, Cm

[21]

 ES05

AJ110637 ΔadhE ΔldhA Δpta ΔbudA ΔpoxB

Km

This work

 ES05/PCK

AJ110637 ΔadhE ΔldhA Δpta ΔbudA ΔpoxB harboring pSTV28-pck

Km, Cm

This work

 ES06

AJ110637 ΔadhE ΔldhA Δpta ΔbudA ΔpoxB::P tac -pck (A. succinogenes)

Km

This work

 ES07

AJ110637 ΔadhE ΔldhA Δpta ΔbudA ΔpoxB::P tac -pck (A. succinogenes) P tac -frdABCD

Km

This work

 ES08

AJ110637 ΔadhE ΔldhA Δpta ΔbudA ΔpoxB::P tac -pck (A. succinogenes) P tac -frdABCD ΔpflB

Km

This work

 ES08ΔptsG

AJ110637 ΔadhE ΔldhA Δpta ΔbudA ΔpoxB::P tac -pck (A. succinogenes) P tac -frdABCD ΔpflB ΔptsG

Km

This work

 ES08ΔptsI

AJ110637 ΔadhE ΔldhA Δpta ΔbudA ΔpoxB::P tac -pck (A. succinogenes) P tac -frdABCD ΔpflB ΔptsI

Km

This work

Plasmid

 pRSFRedTER

Broad-host-range λ Red-expressing plasmid

Cm

[44]

 pMW- attL λ-KmR- attR λ

Cassette for gene disruption containing kanamycin resistance gene

Km

[45]

 pRSF-P ara -IX

Plasmid for removal of antibiotic resistance genes

Cm

[21]

 pSTV28

Plasmid vector with a replication origin of pACYC184

Cm

TAKARA BIO

 pSTV28-pck

Plasmid for expression of pck from A. succinogenes

Cm

[20]

 pSTV28-pck+pyc

Plasmid for co-expression of pck from A. succinogenes and pyc from C. glutamicum

Cm

[21]