Table 1 Plasmids and strains used in this study
Plasmid or strain | Relevant characteristics | Source |
|---|---|---|
Plasmid | ||
pET-22b(+) | f1 ori, T7 promoter, AmpR | Novagen |
pET-28a(+) | f1 ori, T7 promoter, KanR | Novagen |
pET-opTAL | pET-28a(+) carrying codon-optimized tyrosine ammonia lyase gene (tal) | Kang et al. [20] |
pET-Sam5 | pET-28a(+) carrying 4-coumarate 3-hydroxylase gene (sam5) | Choi et al. [17] |
pET-COM | pET-28a(+) carrying caffeic acid methyltransferase gene (com) | Choi et al. [17] |
pET-opT5 | pET-28a(+) carrying tal and sam5 gene | Kang et al. [20] |
pET-opT5M | pET-28a(+) carrying tal, sam5, and com gene | Kang et al. [20] |
pET28-tyrA* | pET-28a(+) carrying feedback-inhibition resistant chorismate mutase/prephenate dehydrogenase gene (tyrAfbr) | Kang et al. [20] |
pET22-aroG* | pET-22b(+) carrying feedback-inhibition resistant DAHP synthase gene (aroGfbr) | Kang et al. [20] |
pET-AG | pET-28a(+) carrying tyrAfbr and aroGfbr gene | This study |
pET22-baPAD | pET-22b(+) carrying codon-optimized phenolic acid decarboxylase gene (pad) | This study |
pET-opTD | pET-28a(+) carrying tal and pad gene | This study |
pET-opT5D | pET-28a(+) carrying tal, sam5 and pad gene | This study |
pET-opT5MD | pET-28a(+) carrying tal, sam5, com and pad gene | This study |
Strain | ||
E. coli DH5α | Cloning host | Invitrogen |
E. coli C41(DE3) | Derivative strain of E. coli BL21(DE3) | Miroux and Walker [31] |
ΔtyrR | tryR gene in-frame deletion mutant of E. coli C41(DE3) | Kang et al. [20] |
ΔCOS1 | E. coli C41(DE3); ΔtyrR::tyrA fbr, aroG fbr; tyrosine overproducing strain | This study |