Figure 5
Phylogenetic tree of PHA synthases. The evolutionary history was inferred using the Neighbor-Joining method [54]. The optimal tree with the sum of branch length = 2.80718513 is shown. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the Poisson correction method [55] and are in the units of the number of amino acid substitutions per site. The analysis involved 32 amino acid sequences. All positions containing gaps and missing data were eliminated. There were a total of 294 positions in the final dataset. Evolutionary analyses were conducted in MEGA5 [56]. Sequences for PhaC1 synthases: P. aeruginosa PAO1 [GenBank:CAA47151.1], P. resinovorans NRRL B-2649 [GenBank:AAD26365.2], P. chlororaphis subsp. aureofaciens [GenBank:BAB78719.1], P. extremaustralis 14-3 [GenBank:CBZ05904.1], Pseudomonas sp. 61-3 [GenBank:BAA36200.1], P. putida KT2440 [GenBank:AAM63407.1], P. stutzeri 1317 [GenBank:AAO59383.1], P. nitroreducens [GenBank:AAL92579.1], Pseudomonas sp. USM4-55 [GenBank:ABX64434.1], P. mendocina ymp [GenBank:AAM10544.1], P. pseudoalcaligenes CECT5344 [GenBank:CDM39028.1] and P. pseudoalcaligenes YS1 [GenBank:AAK95382.1]. Sequences for PhaC2 synthases: P. aeruginosa PAO1 [GenBank:CAA47153.1], P. resinovorans NRRL B-2649 [GenBank:AAD26367.2], Pseudomonas sp. 61-3 [GenBank:BAA36202.1], P. extremaustralis 14-3 [GenBank:CBA13557.1], P. chlororaphis subsp. aureofaciens [GenBank:BAB78721.1], P. putida KT2440 [GenBank:AAM63409.1], P. stutzeri 1317 [GenBank:AAO59384.1], Pseudomonas sp. USM4-55 [GenBank:ABX64435.1], P. mendocina ymp [GenBank:AAM10546.1], P. pseudoalcaligenes CECT5344 [GenBank:CDM39026.1], P. pseudoalcaligenes YS1 [GenBank:AAK95384.1] and P. nitroreducens [GenBank:AAK19608.1]. Sequences for PhaC synthases: Cupriavidus necator VKPM B5786 [GenBank:AAW65074.1], Azotobacter sp. FA8 [GenBank:CAC41638], Pseudomonas sp. 61-3 [GenBank:BAA36198], P. aeruginosa DM2 [GenBank:AEC32220], P. oleovorans NRRL B-778 [GenBank:AAL17612], P. pseudoalcaligenes CECT5344 [GenBank:CDM42645], Pseudomonas sp. USM4-55 [GenBank:ACN22029] and P. extremaustralis 14-3 [GenBank:CAK18904].