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Figure 5 | Microbial Cell Factories

Figure 5

From: Functional display of heterotetrameric human protein kinase CK2 on Escherichia coli: a novel tool for drug discovery

Figure 5

Influence of NaCl on surface displayed human CK2α (filled circle) and surface displayed human CK2α + CK2β (filled square). Increasing concentrations of NaCl ranging from 0 to 300 mM were applied, and the kinase activity of cells either displaying CK2α or CK2α + CK2β were determined by the CE assay. The substrate peptide (RRRDDDSDDD) concentration was 100 µM and the ATP-concentration was 600 µM. Activity was measured for 60 min at 30°C with cells of an OD578 = 2, which corresponds to 1.72 × 108 cells/mL. Samples without NaCl were set to 100% activity.

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Microbial Cell Factories

ISSN: 1475-2859

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